Mouse anti Glial fibrillary acidic protein / GFAP | MyBioSource MBS570104 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti Glial fibrillary acidic protein / GFAP

catalog :

MBS570104

quantity :

0.1 mg

price :

325 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F2

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

catalog number :

MBS570104

products type :

Antibody

products full name :

Mouse anti Glial fibrillary acidic protein / GFAP

products short name :

Glial fibrillary acidic protein / GFAP

other names :

glial fibrillary acidic protein; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein

other gene names :

GFAP; GFAP; FLJ45472; GFAP

uniprot entry name :

GFAP_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

6F2

host :

Mouse

reactivity :

Human

specificity :

6F2 reacts exclusively with glial fibrillary acidic protein which is present in astrocytes in the central nervous system and Schwann cells.

form :

The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.

storage stability :

Store at 4 degree C, or in small aliquots at -20 degree C.

tested application :

Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)

app notes :

6F2 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.

other info1 :

Source Note: 6F2 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with glial fibrillary acidic protein from Human brain.

products categories :

Cytoskeletal, Cancer, Cytoskeleton, Neurobiology, Stem cell research

products description :

GFAP (55 kD) is selectively loCated in astrocytes and represents the major constituent of astrocytic intermediate filaments. GFAP expression levels are highly variable during development of the central nervous system. In adults, GFAP levels increase as a result of the prolifeRation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer's disease, epilepsy and multiple sclerosis. In the peripheral nervous system GFAP is expressed by Schwann cells. Upon differentiation, myelin forming Schwann cells down-regulate GFAP, whereas in non-myelin forming Schwann cells GFAP persists into adulthood.

products references :

Van Muijen, G. N., Ruiter, D. J., and Warnaar, S. O. (1987). Coexpression of intermediate filament polypeptides in Human fetal and adult tissues, Lab Invest 57, 359-69.

ncbi gi num :

251802

uniprot acc num :

P14136

ncbi mol weight :

49,880 Da

ncbi pathways :

Nuclear Signaling By ERBB4 Pathway 530744!!Signal Transduction Pathway 477114!!Signaling By ERBB4 Pathway 530741

ncbi summary :

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

uniprot summary :

Function: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Subunit structure: Interacts with SYNM. By similarity. Isoform 3 interacts with PSEN1 (via N-terminus). Ref.15. Subcellular location: Cytoplasm. Note: Associated with intermediate filaments. Ref.15. Tissue specificity: Expressed in cells lacking fibronectin. Ref.3. Post-translational modification: Phosphorylated by PKN1. Ref.17. Involvement in disease: Defects in GFAP are a cause of Alexander disease (ALEXD) [. MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24. Sequence similarities: Belongs to the intermediate filament family.

size :

0.1 mg

price :

325 USD