antibody

Mouse anti M13 phage coat protein g8p

MBS570069

0.1 mg

325 USD

monoclonal

mouse

nonconjugated

RL-ph2

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Antibody

Mouse anti M13 phage coat protein g8p

M13 phage coat protein g8p

Monoclonal

IgG2a

RL-ph2

Mouse

RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Store at 4 degree C, or in small aliquots at -20 degree C.

Affinity chromatography, Flow Cytometry (FC), Immunocytochemistry (IHC), Western Blot (WB)

RL-ph2 is particularly useful for immunoblotting of sepaRated phage proteins, and is also suitable for detection of M13 phages in multistep immunoblotting procedures, immunocytochemistry, flow cytometry, affinity chromatography and ELISA. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.

Source Note: RL-ph2 is a Mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with isolated M13 phage coat proteins.

Filamentous phage

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over Mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from Human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Meulemans, E. V., Nieland, L. J., Debie, W. H., Ramaekers, F. C., and van Eys, G. J. (1995). Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody, Hum Antibodies Hybridomas 6, 113-8. Meulemans, E. V., Slobbe, R., Wasterval, P., Ramaekers, F. C., and van Eys, G. J. (1994). Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody, J Mol Biol 244, 353-60.

0.1 mg

325 USD