Mouse anti CytokeRatin 18 / KeRatin K18 | MyBioSource MBS570046 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti CytokeRatin 18 / KeRatin K18

catalog :

MBS570046

quantity :

1 mL

price :

265 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RGE53

reactivity :

human, mouse, rat, dog, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

product information

catalog number :

MBS570046

products type :

Antibody

products full name :

Mouse anti CytokeRatin 18 / KeRatin K18

products short name :

CytokeRatin 18 / KeRatin K18

other names :

cytokeratin 18; Keratin, type I cytoskeletal 18; keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein; keratin 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18

other gene names :

KRT18; KRT18; K18; CYK18; CYK18

uniprot entry name :

K1C18_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

RGE53

host :

Mouse

reactivity :

Canine, Chicken, Hamster, Human, Mouse, Rabbit, Rat, Swine, Zebrafish

specificity :

RGE53 reacts exclusively with cytokeRatin 18 which is present in glandular epithelial cells of the digestive, respiRatory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.

form :

Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.

storage stability :

Store at 4 degree C, or in small aliquots at -20 degree C.

tested application :

Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Western Blot (WB)

app notes :

RGE53 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:5 - 1:20 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 - 1:100 for immunoblotting appliCations.

other info1 :

Source Note: RGE53 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal prepaRation of cells.

products categories :

Cytoskeletal, Cell differentiation, Cytoskeleton

products description :

CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

products references :

Ramaekers, F., Huysmans, A., Moesker, O., Kant, A., Jap, P., Herman, C., and Vooijs, P. (1983). Monoclonal antibody to keRatin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology, Lab Invest 49, 353-61. Ramaekers, F. C., Puts, J. J., Moesker, O., Kant, A., Huysmans, A., Haag, D., Jap, P. H., Herman, C. J., and Vooijs, G. P. (1983). Antibodies to intermediate filament proteins in the immunohistochemical identifiCation of Human tumours: an overview, Histochem J 15, 691-713. Puts, J. J., Moesker, O., Kenemans, P., Vooijs, G. P., and Ramaekers, F. C. (1985). Expression of cytokeRatins in early neoplastic epithelial lesions of the uterine cervix, Int J Gynecol Pathol 4, 300-13. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keRatin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of keRatin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). KeRatin expression in cervical cancer, Am J Pathol 141, 497-511. van Leenders, G., Dijkman, H., Hulsbergen-van de Kaa, C., Ruiter, D., and Schalken, J. (2000). DemonstRation of intermediate cells during Human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy, Lab Invest 80, 1251-8.

ncbi gi num :

34037

uniprot acc num :

P05783

ncbi mol weight :

48,058 Da

ncbi pathways :

Caspase Cascade In Apoptosis Pathway (137974); EGFR1 Signaling Pathway (198782); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (516)

ncbi summary :

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

uniprot summary :

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis. Protein type: Cytoskeletal; Nucleolus. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: perinuclear region of cytoplasm; cytoplasm; keratin filament; nucleolus; intermediate filament; microtubule organizing center. Molecular Function: protein binding; structural molecule activity. Biological Process: anatomical structure morphogenesis; viral reproduction; tumor necrosis factor-mediated signaling pathway; intermediate filament cytoskeleton organization and biogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; negative regulation of apoptosis. Disease: Cirrhosis, Familial

size1 :

1 mL

price1 :

265 USD