Rat anti E-selectin | MyBioSource MBS570032 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat anti E-selectin

catalog :

MBS570032

quantity :

1 mL

price :

265 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

UZ7

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

product information

catalog number :

MBS570032

products type :

Antibody

products full name :

Rat anti E-selectin

products short name :

E-selectin

other names :

E-selectin; E-selectin; E-selectin; ELAM-1; OTTHUMP00000032745; OTTHUMP00000032746; OTTHUMP00000032747; OTTHUMP00000032748; OTTHUMP00000032749; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; selectin E; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2

other gene names :

SELE; SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2; ELAM1

uniprot entry name :

LYAM2_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

UZ7

host :

Rat

reactivity :

Mouse

specificity :

UZ7 recognizes a conformational epitope in the CR1 region in murine E-selectin. UZ7 partially inhibits leukocyte adhesion to murine activated endothelial cells.

form :

Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.

storage stability :

Store at 4 degree C, or in small aliquots at -20 degree C.

tested application :

Flow Cytometry (FC), Immunocytochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP), Western Blot (WB)

app notes :

UZ7 is suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on fozen sections and flow cytometry (dilution 1:25 - 1:50).

other info1 :

Source Note: UZ7 is a Rat monoclonal IgG2a antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Lewis Rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.

products categories :

Cell adhesion, Cell adhesion, Immunology

products description :

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkappaB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

products references :

Hallmann, R., Zimmermann, U., Sorokin, L. M., Needham, L., and Von der Mark, K. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9. Hammel, M., Weitz-Schmidt, G., Krause, A., Moll, T., Vestweber, D., Zerwes, H. G., and Hallmann, R. (2001). Species-specific and conserved epitopes on Mouse and Human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74.

ncbi gi num :

187960042

ncbi acc num :

NP_000441.2

ncbi gb acc num :

NM_000450.2

uniprot acc num :

P16581

ncbi mol weight :

66,655 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Glucocorticoid Receptor Regulatory Network Pathway (138014); Malaria Pathway (152665); Malaria Pathway (152657); Thromboxane A2 Receptor Signaling Pathway (138060)

ncbi summary :

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq]

uniprot summary :

SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q22-q25. Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; plasma membrane; integral to membrane; coated pit; caveola; lipid raft. Molecular Function: protein binding; phospholipase binding; transmembrane receptor activity; sialic acid binding. Biological Process: positive regulation of leukocyte migration; positive regulation of receptor internalization; actin filament-based process; calcium-mediated signaling; response to lipopolysaccharide; leukocyte migration during inflammatory response; heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; regulation of inflammatory response; leukocyte tethering or rolling; inflammatory response; blood coagulation; leukocyte migration. Disease: Hypertension, Essential

size1 :

1 mL

price1 :

265 USD