antibody

Mouse anti CytokeRatin 18 / KeRatin K18

MBS570030

0.1 mg

330 USD

monoclonal

mouse

nonconjugated

DE-K18

human, dog, zebrafish

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Antibody

Mouse anti CytokeRatin 18 / KeRatin K18

CytokeRatin 18 / KeRatin K18

cytokeratin 18; Keratin, type I cytoskeletal 18; keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein; keratin 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18

KRT18; KRT18; K18; CYK18; CYK18

K1C18_HUMAN

Monoclonal

IgG1

DE-K18

Mouse

Canine, Feline, Human, Zebrafish

DE-K18 reacts exclusively with cytokeRatin 18 which is present in glandular epithelial cells of the digestive, respiRatory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Store at 4 degree C, or in small aliquots at -20 degree C.

Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)

DE-K18 is useful for immunocytochemistry, immunoblotting, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues. For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1 N HCl 30 min at room tempeRature is required. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.

Source Note: DE-K18 is a Mouse monoclonal IgG1, k antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a cytoskeletal prepaRation extracted from the Human vulvar squamous carcinoma cell line A431.

Cytoskeletal, Cell differentiation, Cytoskeleton

CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline cytokeRatins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. Vos, J. H., van den Ingh, T. S., de Neijs, M., van Mil, F. N., Ivanyi, D., and Ramaekers, F. C. (1992). Immunohistochemistry with keRatin monoclonal antibodies in canine tissues: urogenital tract, respiRatory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40. Vos, J. H., van den Ingh, T. S., Ramaekers, F. C., Molenbeek, R. F., de Neijs, M., van Mil, F. N., and Ivanyi, D. (1993). The expression of keRatins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region, Vet Pathol 30, 352-61. Bonfrer, J. M., Groeneveld, E. M., Korse, C. M., van Dalen, A., Oomen, L. C., and Ivanyi, D. (1994). Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantifiCation of tissue polypeptide antigen recognizes keRatin 18, Tumour Biol 15, 210-22.

34037

P05783

48,058 Da

Caspase Cascade In Apoptosis Pathway (137974); EGFR1 Signaling Pathway (198782); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (516)

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis. Protein type: Cytoskeletal; Nucleolus. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: perinuclear region of cytoplasm; keratin filament; cytoplasm; nucleolus; intermediate filament; microtubule organizing center. Molecular Function: protein binding; structural molecule activity. Biological Process: anatomical structure morphogenesis; viral reproduction; tumor necrosis factor-mediated signaling pathway; intermediate filament cytoskeleton organization and biogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; negative regulation of apoptosis. Disease: Cirrhosis, Familial

0.1 mg

330 USD