Mouse anti K-Cadherin/Cadherin-6 | MyBioSource MBS570007 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti K-Cadherin/Cadherin-6

catalog :

MBS570007

quantity :

0.1 mg

price :

360 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2B6

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS570007

products type :

Antibody

products full name :

Mouse anti K-Cadherin/Cadherin-6

products short name :

K-Cadherin/Cadherin-6

other names :

cadherin-6 preproprotein; Cadherin-6; cadherin-6; OTTHUMP00000115839; OTTHUMP00000220908; cadherin 6, type 2, K-cadherin (fetal kidney); Kidney cadherin

other gene names :

CDH6; CDH6; CAD6; KCAD; FLJ14176; DKFZp564N1116

uniprot entry name :

CADH6_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

2B6

host :

Mouse

reactivity :

Human, Rat

specificity :

2B6 recognizes the extracellular domain of cadherin-6.

form :

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

storage stability :

Store at 4 degree C, or in small aliquots at -20 degree C.

tested application :

Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)

app notes :

2B6 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. 2B6 is also suitable for and immunohistochemistry on praraffin-embedded sections. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting appliCations.

other info1 :

Source Note: 2B6 is a Mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with affinity purified extracellular domain of Human cadherin-6-GST fusion protein.

products categories :

Cell adhesion, Cell adhesion

products description :

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the Catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.

products references :

Shimazui, T., Oosterwijk, E., Akaza, H., Bringuier, P., Ruijter, E., van Berkel, H., Wakka, J. O., van Bokhoven, A., Debruyne, F. M., and Schalken, J. A. (1998). Expression of cadherin-6 as a novel diagnostic tool to predict prognosis of patients with E-cadherin-absent renal cell carcinoma, Clin Cancer Res 4, 2419-24. Shimazui, T., Oosterwijk-Wakka, J., Akaza, H., Bringuier, P. P., Ruijter, E., Debruyne, F. M., Schalken, J. A., and Oosterwijk, E. (2000). AlteRations in expression of cadherin-6 and E-cadherin during kidney development and in renal cell carcinoma, Eur Urol 38, 331-8.

ncbi gi num :

4826673

ncbi acc num :

NP_004923.1

ncbi gb acc num :

NM_004932.3

uniprot acc num :

P55285

ncbi mol weight :

88,309 Da

ncbi pathways :

Adherens Junctions Interactions Pathway 119533!!Cell Junction Organization Pathway 160966!!Cell-cell Junction Organization Pathway 119532

ncbi summary :

This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis. [provided by RefSeq]

uniprot summary :

Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Subcellular location: Cell membrane; Single-pass type I membrane protein. Tissue specificity: Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas. Sequence similarities: Contains 5 cadherin domains.

size :

0.1 mg

price :

360 USD

more info or order :

MyBioSource product webpage