Mouse anti Lamin B1 | MyBioSource MBS570006 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti Lamin B1

catalog :

MBS570006

quantity :

1 ml

price :

265 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

119D5-F1

reactivity :

human, mouse, rat, dog, cow, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

catalog number :

MBS570006

products type :

Antibody

products full name :

Mouse anti Lamin B1

products short name :

Lamin B1

other names :

lamin B1; Lamin-B1; lamin-B1; OTTHUMP00000159218; lamin B1

other gene names :

LMNB1; LMNB1; LMN; ADLD; LMN2; LMNB; MGC111419; LMN2; LMNB

uniprot entry name :

LMNB1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

119D5-F1

host :

Mouse

reactivity :

Bovine, Canine, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

specificity :

119D5-F1 reacts with an epitope loCated C-terminal of residue 231 in lamin B1.

form :

Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.

storage stability :

Store at 4 degree C, or in small aliquots at -20 degree C.

tested application :

Flow Cytometry (FC), Immunocytochemistry (IHC), Western Blot (WB)

app notes :

119D5-F1 is suitable for immunocytochemistry, immunoblotting, ELISA and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:5 - 1:20 for flow cytometry, and for immunocytochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 - 1:100 for immunoblotting appliCations.

other info1 :

Source Note: 119D5-F1 is a Mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with purified Rat liver lamins.

products categories :

Nuclear proteins, Cytoskeleton

products description :

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

products references :

Weaver, V. M., Carson, C. E., Walker, P. R., Chaly, N., Lach, B., Raymond, Y., Brown, D. L., and Sikorska, M. (1996). Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes, J Cell Sci 109, 45-56. Pugh, G. E., Coates, P. J., Lane, E. B., Raymond, Y., and Quinlan, R. A. (1997). Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells, J Cell Sci 110, 2483-93. Broers, J. L., Machiels, B. M., Kuijpers, H. J., Smedts, F., van den Kieboom, R., Raymond, Y., and Ramaekers, F. C. (1997). A- and B-type lamins are differentially expressed in normal Human tissues, Histochem Cell Biol 107, 505-17. Machiels, B. M., Broers, J. L., Raymond, Y., de Ley, L., Kuijpers, H. J., Caberg, N. E., and Ramaekers, F. C. (1995). Abnormal A-type lamin organization in a Human lung carcinoma cell line, Eur J Cell Biol 67, 328-35. Machiels, B. M., Ramaekers, F. C., Kuijpers, H. J., Groenewoud, J. S., Oosterhuis, J. W., and Looijenga, L. H. (1997). Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults, J Pathol 182, 197-204. Jansen, M. P., Machiels, B. M., Hopman, A. H., Broers, J. L., Bot, F. J., Arends, J. W., Ramaekers, F. C., and Schouten, H. C. (1997). Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease, Histopathology 31, 304-12. Neri, L. M., Raymond, Y., Giordano, A., Capitani, S., and Martelli, A. M. (1999). Lamin A is part of the internal nucleoskeleton of Human erythroleukemia cells, J Cell Physiol 178, 284-95. Broers, J. L., Bronnenberg, N. M., Kuijpers, H. J., Schutte, B., Hutchison, C. J., and Ramaekers, F. C. (2002). Partial cleavage of A-type lamins concurs with their total disintegRation from the nuclear lamina during apoptosis. Eur J Cell Biol 81, 677-691.

ncbi gi num :

576840

uniprot acc num :

P20700

ncbi mol weight :

66,408 Da

ncbi pathways :

Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Breakdown Of The Nuclear Lamina Pathway 105681!!Caspase Cascade In Apoptosis Pathway 137974!!FAS Pathway And Stress Induction Of HSP Regulation 198894

ncbi summary :

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq]

uniprot summary :

Function: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Subunit structure: Interacts with lamin-associated polypeptides IA, IB and 2. Subcellular location: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side. Post-translational modification: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Involvement in disease: Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [. MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. Ref.12. Miscellaneous: The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. Sequence similarities: Belongs to the intermediate filament family.

size :

1 ml

price :

265 USD