antibody

HSC70/HSP73 polyclonal antibody

MBS565298

0.05 mg

255 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry

Antibody

HSC70/HSP73 polyclonal antibody

[HSC70/HSP73]

[HSC70/HSP73]

[Heat shock cognate 71 kDa protein; Heat shock cognate 71 kDa protein; heat shock cognate 71 kDa protein; heat shock protein family A (Hsp70) member 8; Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LPS-associated protein 1]

[Heat shock protein 70; Hsc70; Hsp73]

[HSPA8; HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p; HSC70; HSP73; HSPA10; LAP-1; LPS-associated protein 1]

HSP7C_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep

646

Recognizes human, mouse, rat, bovine, dog, guinea pig, hamster, monkey, pig, rabbit and sheep HSC70 (HSP73). Detects a band of ~73kDa by Western blot.

Protein A affinity purified.

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Long term storage: -20°C. Avoid freeze/thaw cycles. Shipped on blue ice.

Immunohistochemistry (IHC) (PS), Western Blot (WB)

Western Blot (1:1,000, colorimetric). Suggested dilutions/conditions may not be available for all applications. Detects a band of ~73kDa by Western blot.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunogen: Synthetic peptide corresponding to a portion of human Hsc70 (Hsp73). The sequence is completely conserved in rat, mouse, hamster and bovine.

Protein Synthesis & Folding

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

123648

P11142.1

P11142

AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway (1269726); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Attenuation Phase Pathway (1270424); Axon Guidance Pathway (1270303); C-MYB Transcription Factor Network Pathway (138073); CHL1 Interactions Pathway (1270327); Cellular Response To Heat Stress Pathway (1270421); Cellular Responses To Stress Pathway (1270414); Clathrin Derived Vesicle Budding Pathway (1269881)

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleolus; Heat shock protein; Chaperone; RNA-binding. Chromosomal Location of Human Ortholog: 11q24.1. Cellular Component: cytosol; extracellular space; focal adhesion; intracellular; lysosomal lumen; lysosomal membrane; melanosome; membrane; myelin sheath; nucleolus; nucleoplasm; nucleus; plasma membrane; ribonucleoprotein complex; spliceosome; ubiquitin ligase complex. Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; protein binding; ubiquitin protein ligase binding; unfolded protein binding. Biological Process: ATP metabolic process; axon guidance; cellular response to starvation; chaperone cofactor-dependent protein folding; gene expression; negative regulation of transcription, DNA-dependent; neurotransmitter secretion; nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; post-Golgi vesicle-mediated transport; protein folding; protein refolding; protein targeting to lysosome; regulation of cell cycle; regulation of mRNA stability; regulation of protein complex assembly; regulation of protein stability; response to unfolded protein; RNA splicing; synaptic transmission; transcription, DNA-dependent; viral reproduction

0.05 mg

255 USD

0.2 mg

475

1 mg

1705