ELISA/assay

Hamster Phospholipase B-Like 2 (PLBL2) ELISA Kit

MBS564202

1 Plate

1100 USD

ELISA Kit

Hamster Phospholipase B-Like 2 (PLBL2) ELISA Kit

[Phospholipase B-Like 2]

[PLBL2]

[PLBL2]

This ELISA is specific for the measurement of natural and recombinant human GPX3. It does not cross-react with human adiponectin, human resistin, human FABP3, human FABP4, human ACE2, human IL-6, human SOCS-3, human 4-1BB ligand, human ANGPTL4, human Th-pok, human Foxp3, human ANGPTL6, human IDO.

Samples: Serum, Plasma And Cell Culture Supernatant. Assay Type: Sandwich. Detection Range: 0.5 ng/ml-32 ng/ml. Sensitivity: 100 pg/ml

Intra-assay Precision: Eight serum samples of known concentrations of human GPX3 were assayed in replicates 12 times to test precision within an assay. Inter-assay Precision: Eight samples of known concentrations of human GPX3 were assayed in 8 separate assays to test precision between assays.

Intended Uses: The GPX3 human ELISA Kit is to be used for the measurement of human GPX3 in serum, plasma and cell culture supernatant. This ELISA Kit is for research use only. Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of human GPX3 in biological fluids. A polyclonal antibody specific for GPX3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, GPX3 is recognized by the addition of a biotinylated polyclonal antibody specific for GPX3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (STREPHRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of GPX3 in the samples!!Background/Introduction: Glutathione peroxidase (GPX) catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. This enzyme, found mainly in the cytosol of mammalian cells, is unusual in its content of a selenocysteine residue in its active site that is encoded by a TGA (stop codon) (1). The glutathione peroxidase found in plasma now called GPX3 is immunologically distinct from the erythrocyte and liver cytosolic enzymes. It also has some differences in physical and kinetic properties. Takahashi et al. (2) isolated cDNA clones coding for plasma GPX3. They found that the nucleotide sequence consisted of a 678-bp open reading frame coding for a 226-amino acid polypeptide with a molecular mass of 25,389. The amino acid sequence showed only 44% homology with other human cellular GPX family. Takahashi et al. (2) concluded that as the plasma enzyme contains 1 atom of selenium per subunit, the in-frame TGA observed at positions 217-219 could be assigned to selenocysteine and a tetramer of approximately 90-100 kDa where each of the 4 identical subunits contains an active site with the selenium atom in the form of selenocysteine residue (2). GPX3 is also found in human milk (3). Chu et al. (4) found that glutathione peroxidase-3 is expressed in kidney, lung, heart, breast, placenta, and, in the human but not the rodent, in liver as well. Since redox control has been implicated in the cause of metabolic dysfunction, plasma or serum measurement of GPX3 may give some benefits to the investigation of these metabolic diseases.

1 Plate

1100 USD