catalog number :
MBS564199
products type :
ELISA Kit
products full name :
Dog KIM-1
products short name :
KIM-1
other names :
kidney injury molecule-1; Hepatitis A virus cellular receptor 1 homolog; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; TIMD-1
products gene name :
KIM-1
other gene names :
Havcr1; Kim1; HAVcr-1; KIM-1; TIMD-1
uniprot entry name :
HAVR1_RAT
other info1 :
Samples: Biological fluid of Dogs. Assay Type: Sandwich
other info2 :
Intended Uses: The Kidney Injury Molecule-1 (KIM-1) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring KIM-1 in biological fluid of Dogs.
products description :
Principle of the assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
ncbi acc num :
AAC53546.1
ncbi mol weight :
33,964 Da