ELISA/assay

Sheep Haptoglobin

MBS564186

1 x96 Wells

520 USD

ELISA Kit

Sheep Haptoglobin

Haptoglobin

haptoglobin; Haptoglobin; haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide; haptoglobin; ZonulinCleaved into the following 2 chains:Haptoglobin alpha chain; Haptoglobin beta chain

HP; HP; BP; HPA1S; HP2ALPHA2

HPT_HUMAN

Sheep

406

Samples: Biological fluid of Sheep. Assay Type: Sandwich

Intended Uses: The Haptoglobin (HPT) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring HPT in biological fluid of Sheep.

Principle of the assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the HPT present in samples reacts with the anti-HPT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HPT antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound HPT. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HPT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HPT in the test sample. The quantity of HPT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

386783

AAA88080.1

38,452 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600); Amb2 Integrin Signaling Pathway (137945)

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16q22.2. Cellular Component: extracellular space; extracellular region. Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity. Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response. Disease: Anhaptoglobinemia

1 x96 Wells

520 USD