catalog number :
MBS564154
products type :
ELISA Kit
products full name :
Human Osteoprotegrin ELISA Kit
products short name :
Osteoprotegrin
products name syn :
Human Osteoprotegrin
storage stability :
Store at 4 degree C.
other info1 :
Samples: Immunoperoxidase Assay for Determination of Osteoprotegrin in Human Samples. Intended Uses: The Osteoprotegrin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Osteoprotegrin in Human biological samples.
products categories :
ELISA Kit
products description :
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Osteoprotegrin present in samples reacts with the anti-Osteoprotegrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Osteoprotegrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Osteoprotegrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Osteoprotegrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Osteoprotegrin in the test sample. The quantity of Osteoprotegrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.