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ELISA/assay

Human CXCL10 (IP-10) ELISA Kit

MBS564153

1 x96 Wells

520 USD

MBS564153

ELISA Kit

Human CXCL10 (IP-10) ELISA Kit

CXCL10 (IP-10)

Human CXCL10 (IP-10)

C-X-C motif chemokine 10; C-X-C motif chemokine 10; C-X-C motif chemokine 10; gamma IP10; gamma-IP10; OTTHUMP00000219215; small-inducible cytokine B10; interferon-inducible cytokine IP-10; 10 kDa interferon gamma-induced protein; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10; 10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; Small-inducible cytokine B10

CXCL10; CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10; INP10; SCYB10

CXL10_HUMAN

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of CXCL10 in Human Samples. Intended Uses: The CXCL10 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CXCL10 in biological samples of human.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CXCL10 present in samples reacts with the anti-CXCL10 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CXCL10 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CXCL10. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CXCL10 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CXCL10 in the test sample. The quantity of CXCL10 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

149999382

NP_001556.2

NM_001565.3

P02778

10,881 Da

CXCR3-mediated Signaling Events Pathway 138011!!Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytosolic DNA-sensing Pathway 125137!!Cytosolic DNA-sensing Pathway 124833!!G Alpha (i) Signalling Events Pathway 119550

This gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq]

Function: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Subcellular location: Secreted. Induction: By IFNG/IFN-gamma. A diverse population of cell types rapidly increases transcription of mRNA encoding this protein. This suggests that gamma-induced protein may be a key mediator of the IFNG/IFN-gamma response. Post-translational modification: CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family. Mass spectrometry: Molecular mass is 8641.8 Da from positions 22 - 98. Determined by ESI. Ref.5

1 x96 Wells

520 USD