catalog number :
MBS564147
products type :
ELISA Kit
products full name :
Bovine IgM ELISA Kit
products short name :
Bovine IgM
storage stability :
Store at 4 degree C.
other info1 :
Samples: Immunoperoxidase Assay for Determination of IgM in Bovine Samples. Assay Type: Sandwich
other info2 :
Intended Uses: The total Bovine IgM test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM in bovine biological samples.
products categories :
ELISA Kit
products description :
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)