ELISA/assay

Dog NGAL ELISA Kit

MBS564146

1 x96 Wells

520 USD

ELISA Kit

Dog NGAL ELISA Kit

NGAL (Lipocalin-2)

Dog NGAL (Lipocalin-2)

neutrophil gelatinase-associated lipocalin; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; siderocalin; oncogene 24p3; OTTHUMP00000022238; OTTHUMP00000022239; OTTHUMP00000022240; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; p25

LCN2; LCN2; 24p3; MSFI; NGAL; HNL; NGAL

NGAL_HUMAN

Dog

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of NGAL in Dog Samples. Assay Type: Sandwich

Intended Uses: The NGAL test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring NGAL in biological samples of dogs.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the NGAL present in samples reacts with the anti-NGAL antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-NGAL, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of NGAL in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of NGAL in the test sample. The quantity of NGAL in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

38455402

NP_005555.2

NM_005564.3

P80188

22,588 Da

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding. Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; positive regulation of cell projection organization and biogenesis; siderophore transport; innate immune response; ion transport; response to herbicide; transmembrane transport

1 x96 Wells

520 USD