ELISA/assay

Human Hemoglobin ELISA Kit

MBS564144

1 x96 Wells

470 USD

ELISA Kit

Human Hemoglobin ELISA Kit

Hemoglobin

hemoglobin; hemoglobin; GRMZM2G067402; non-symbiotic hemoglobin; reversible oxygen binding

LOC541815

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Hemoglobin in Human Samples. Assay Type: Sandwich

Intended Uses: The Hemoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemoglobin in serum or plasma samples of humans.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hemoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemoglobin in the test sample. The quantity of Hemoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Hemoglobin is a water-soluble, iron storage protein. Serum Hemoglobin level is said to be useful for diagnosis of iron deficiency anemia, metabolism disorder and malignant tumor. Hemoglobin may also be an acute-phase protein and is often elevated in the course of disease.

162462053

NP_001104966.1

NM_001111496.1

1 x96 Wells

470 USD