ELISA/assay

Human NGAL ELISA Kit

MBS564140

1 x96 Wells

530 USD

ELISA Kit

Human NGAL ELISA Kit

[NGAL (Lipocalin-2)]

[Human NGAL (Lipocalin-2)]

[Lipocalin 2; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; siderocalin; oncogene 24p3; OTTHUMP00000022238; OTTHUMP00000022239; OTTHUMP00000022240; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; p25]

[LCN2; LCN2; 24p3; MSFI; NGAL; HNL; NGAL]

NGAL_HUMAN

Human

This ELISA is specific for the measurement of natural and recombinant mouse Lipocalin-2. It does not cross-react with human Lipocalin-2.

Store at 4 degree C.

Samples: Cell Culture Supernatants, Serum And Plasma. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.1 ng/ml

Intra-assay Precision: C.V <7%. Inter-assay Precision: C.V <12%

ELISA Kit

Intended Uses: The Lipocalin-2 mouse ELISA kit is to be used for the measurement of mouse Lipocalin-2 in cell culture supernatants, serum and plasma. This ELISA kit is for research use only. Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of mouse Lipocalin-2 in cell culture supernatants, serum and plasma. A polyclonal antibody specific for Lipocalin-2 has been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Lipocalin-2 is recognized by the addition of a biotinylated polyclonal antibody specific for mouse Lipocalin-2 (DET). After removal of excess biotinylated antibody, streptavidin-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of mouse Lipocalin-2 in the samples!!Background/Introduction: Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), 24p3, or neutrophil lipocalin (NL), is a 25-kDa secretory glycoprotein (1). Lipocalin 2 was initially defined as a powerful bacteriostatic agent active against various Gramnegative microorganisms through impeding bacterial iron sequestration (2, 3). LCN2 also functions as a stress protein that is released in a variety of other sterile inflammatory conditions such as adipose obesity-related inflammation and cancer (2, 3). LCN2 is over-expressed in many types of cancers including breast, pancreatic, and ovarian carcinomas and promotes tumorigenesis through enhancing tumor cell survival and proliferation, and metastatic potential (4, 5, 6). Furthermore, a growing body of evidence suggests that serum levels of lipocalin-2 are correlated with obesity, insulin resistance, hyperglycemia, coronary heart disease, acute renal injury and fatty liver disease (7, 8, 9).

21619839

P80188

22,588 Da

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding. Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; positive regulation of cell projection organization and biogenesis; siderophore transport; innate immune response; ion transport; response to herbicide; transmembrane transport

1 x96 Wells

530 USD