ELISA/assay

Rat KIM-1 ELISA Kit

MBS564137

1 x96 Wells

545 USD

ELISA Kit

Rat KIM-1 ELISA Kit

KIM-1

Rat KIM-1

kidney injury molecule-1

Rat

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Kim-1 in Rat Samples. Assay Type: Sandwich

Intended Uses: The Kidney Injury Molecule (KIM-1) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring KIM-1 in biological samples of rats.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-KIM-1 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound KIM-1. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: KIM-1 is a trans-membrane structural glycoprotein in renal epithelial cells. These cells will shed KIM-1 antigen during regeneration into the urine which makes urinary KIM-1 a useful biomarker for kidney injury.

2665892

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

1 x96 Wells

545 USD