catalog number :
MBS564131
products type :
ELISA Kit
products full name :
Pig IgM ELISA Kit
products short name :
[Pig IgM]
storage stability :
Store at 4 degree C.
other info1 :
Samples: Human Biological Samples. Assay Type: Sandwich
products categories :
ELISA Kit
products description :
Intended Uses: The total Human IgO test kit is a highly sensitive twosite enzyme linked immunoassay (ELISA) for measuring IgO in human biological samples. Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgO present in samples reacts with the anti-lgO antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-lgO antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgO. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgO in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgO in the test sample. The quantity of IgO in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)