ELISA/assay

Rat Vitamin D Binding Protein ELISA Kit (GC Globulin)

MBS564130

1 x96 Wells

520 USD

ELISA Kit

Rat Vitamin D Binding Protein ELISA Kit (GC Globulin)

Vitamin D Binding Protein

Rat Vitamin D Binding Protein

vitamin D-binding protein isoform 1; Vitamin D-binding protein; vitamin D-binding protein; VDB; gc-globulin; OTTHUMP00000160364; OTTHUMP00000218973; OTTHUMP00000218974; OTTHUMP00000218975; vitamin D-binding alpha-globulin; group-specific component (vitamin D binding protein); Gc-globulin; Group-specific component

GC; GC; DBP; GRD3; VDBG; VDBP; DBP/GC

VTDB_HUMAN

Rat

Samples: Immunoperoxidase Assay for Determination of Vitamin D Binding Protein in Rat Samples. Assay Type: Sandwich

Intended Uses: The total Rat Vitamin D Binding Protein (VDBP) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring VDBP in biological samples of Rats.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the VDBP present in samples reacts with the anti-VDBP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-VDBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound VDBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of VDBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of VDBP in the test sample. The quantity of VDBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution

32483410

NP_000574.2

NM_000583.3

P02774

52,964 Da

Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamins A And D Pathway (106150); Metabolism Of Vitamins And Cofactors Pathway (106249); Vitamin D (calciferol) Metabolism Pathway (160956)

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq]

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q12-q13. Cellular Component: extracellular space; lysosomal lumen; extracellular region; cytosol. Molecular Function: vitamin D binding; actin binding; vitamin transporter activity. Biological Process: steroid metabolic process; vitamin transport; vitamin D metabolic process. Disease: Graves Disease, Susceptibility To, 1

1 x96 Wells

520 USD