ELISA/assay

Rat Transferrin ELISA Kit

MBS564129

1 x96 Wells

330 USD

ELISA Kit

Rat Transferrin ELISA Kit

Transferrin

Rat Transferrin

transferrin; Serotransferrin; serotransferrin; siderophilin; OTTHUMP00000197155; beta-1 metal-binding globulin; transferrin; Beta-1 metal-binding globulin; Siderophilin

TF; TF; PRO1557; PRO2086; DKFZp781D0156

TRFE_HUMAN

Rat

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Transferrin in Rat Samples. Assay Type: Sandwich

Intended Uses: The Transferrin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Transferrin in biological samples of rats.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Transferrin present in samples reacts with the anti-Transferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-TX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound TX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of TX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of TX in the test sample. The quantity of TX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Transferrin (TX) is a metal-combining protein that reversibly binds to acid-soluble iron in plasma. It functions to transport iron to the bone marrow and to tissue storage organs such as the liver. Transferrin also participate in the regulation and control of iron absorption and protects against iron intoxication. Like haptoglobin, the carrier of hemoglobin, transferrin is synthesized in the liver, but unlike haptoglobin transferrin is returned to the circulation after unloading its iron in the reticuloendothelial system. This ELISA kit can be used to measure transferrin in serum, tissue extracts and other biological fluids.

248648

P02787

77,064 Da

EPHB Forward Signaling Pathway (138047); Formation Of Platelet Plug Pathway (106029); HIF-1-alpha Transcription Factor Network Pathway (138045); Hemostasis Pathway (106028); Iron Uptake And Transport Pathway (187191); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Platelet Activation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq]

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q22.1. Cellular Component: extracellular space; cell surface; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; coated pit; recycling endosome; perinuclear region of cytoplasm; endocytic vesicle; apical plasma membrane; late endosome; basal plasma membrane; endosome membrane; vesicle. Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding. Biological Process: platelet activation; retinal homeostasis; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport. Disease: Atransferrinemia

1 x96 Wells

330 USD