product summary
request information :
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat SAP ELISA Kit
catalog :
MBS564127
quantity :
1 x96 Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS564127
products type :
ELISA Kit
products full name :
Rat SAP ELISA Kit
products short name :
SAP
products name syn :
Rat SAP
other names :
serum amyloid P-component; Serum amyloid P-component; serum amyloid P-component; SAP; N/A
other gene names :
APCS; APCS; MGC127839; N/A
uniprot entry name :
SAMP_BOVIN
host :
Rat
storage stability :
Store at 4 degree C.
other info1 :
Samples: Immunoperoxidase Assay for Determination of SAP in Rat Samples. Intended Uses: The SAP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAP in biological samples of rats.
products categories :
ELISA Kit
products description :
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the SAP present in samples reacts with the anti-SAP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAP in the test sample. The quantity of SAP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Rat serum amyloid P (SAP) is an acute phase protein that circulates as a ~230 kDa glycoprotein. It is composed of 10 identical subunits arranged as two cyclic pentameric discs and contains ~10% carbohydrate. Measurement of the concentration of SAP is indicative of the extent and severity of an inflammatory stimulus and can be used to assess various modalities of treatment.
ncbi gi num :
77735883
ncbi acc num :
NP_001029638.1
ncbi gb acc num :
NM_001034466.1
uniprot acc num :
Q3T004
ncbi mol weight :
25,183 Da
uniprot summary :
Cofactor: Binds 2 calcium ions per subunit. Subunit structure: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits. Subcellular location: Secreted. Involvement in disease: Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Sequence similarities: Belongs to the pentaxin family.Contains 1 pentaxin domain.
size :
1 x96 Wells
price :
470 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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