product summary
request information :
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Myoglobin ELISA Kit
catalog :
MBS564122
quantity :
1 x96 Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS564122
products type :
ELISA Kit
products full name :
Rat Myoglobin ELISA Kit
products short name :
Myoglobin
products name syn :
Rat Myoglobin
other names :
myoglobin; Myoglobin; myoglobin; OTTHUMP00000197919; OTTHUMP00000197921; OTTHUMP00000197922; OTTHUMP00000197935; myoglobin; N/A
other gene names :
MB; MB; PVALB; MGC13548; N/A
uniprot entry name :
MYG_HUMAN
host :
Rat
storage stability :
Store at 4 degree C.
other info1 :
Intended Uses: The Myoglobin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Myoglobin in biological samples of rats.
products categories :
ELISA Kit
products description :
Principle of the assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Myoglobin present in samples reacts with the anti- Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti- Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzymelabeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Introduction: Myoglobin is a single-chain globular protein containing a heme group in the center. Myoglobin is found in both cardiac and skeletal muscle tissues. It is released from damaged mucsle tissues and therefore makes it a sensitive marker for muscle injury.
ncbi gi num :
4885477
ncbi acc num :
NP_005359.1
ncbi gb acc num :
NM_005368.2
uniprot acc num :
P02144
ncbi mol weight :
17,184 Da
ncbi summary :
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]
uniprot summary :
Function: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Sequence similarities: Belongs to the globin family.
size :
1 x96 Wells
price :
470 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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