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ELISA/assay

Rat Hemopexin ELISA Kit

MBS564116

1 x96 Wells

470 USD

MBS564116

ELISA Kit

Rat Hemopexin ELISA Kit

Hemopexin

Rat Hemopexin

hemopexin; Hemopexin; hemopexin; beta-1B-glycoprotein; hemopexin; Beta-1B-glycoprotein

HPX; HPX; HX; FLJ56652; HPX; N/A

HEMO_HUMAN

Rat

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Hemopexin in Rat Samples. Intended Uses: The Hemopexin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin in biological samples of rats.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Hemopexin (HX) is the plasma protein with the highest binding affinity to heme among known proteins. It is mainly expressed in liver and belongs to acute phase reactants, the synthesis of which is induced after inflammation. This ELISA kit can be used to measure Hemopexin in serum, tissue extracts and other biological fluids.

184497

P02790

51,676 Da

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq]

Function: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Subunit structure: Interacts with HEV ORF3 protein. Interacts with FLVCR1. Ref.12 Ref.15. Subcellular location: Secreted. Tissue specificity: Expressed by the liver and secreted in plasma. Post-translational modification: N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14. Miscellaneous: The isolated N-terminal domain binds one heme. The full-length protein also binds one heme, but at a different site. The physiological significance of this is not clear. Sequence similarities: Belongs to the hemopexin family.Contains 5 hemopexin-like domains. Sequence caution: The sequence CAA26382.1 differs from that shown. Reason: Erroneous initiation.

1 x96 Wells

470 USD