product summary
request information :
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Hemoglobin ELISA Kit
catalog :
MBS564115
quantity :
1 x96 Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS564115
products type :
ELISA Kit
products full name :
Rat Hemoglobin ELISA Kit
products short name :
Hemoglobin
products name syn :
Rat Hemoglobin
other names :
hemoglobin; hemoglobin; N/A
other gene names :
RB1909; N/A
host :
Rat
storage stability :
Store at 4 degree C.
other info1 :
Samples: Immunoperoxidase Assay for Determination of Hemoglobin in Rat Samples. Intended Uses: The Hemoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemoglobin in biological samples of rats.
products categories :
ELISA Kit
products description :
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HM in the test sample. The quantity of HM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Hemoglobin (HM) is the iron-containing oxygen transport protein in the red blood cells. It picks up oxygen in the lungs and delivers it to the tissues to maintain the viability of cells. Each subunit of hemoglobin is a globular protein with an embedded heme group. Each heme group contains an iron atom and is responsible for the binding of oxygen. Decrease of hemoglobin leads to symptoms of anemia.
ncbi gi num :
32471656
ncbi acc num :
NP_864649.1
ncbi gb acc num :
NC_005027.1
size :
1 x96 Wells
price :
470 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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