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ELISA/assay

Rat Fibrinogen ELISA Kit

MBS564110

1 x96 Wells

415 USD

MBS564110

ELISA Kit

Rat Fibrinogen ELISA Kit

Fibrinogen

Rat Fibrinogen

fibrinogen; Fibrinogen gamma-B chain; fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide; N/A

FGG; FGG; MGC127951; N/A

FIBG_BOVIN

Rat

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Fibrinogen in Rat Samples. Intended Uses: The Fibrinogen test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of rats.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Soluble Fibrinogen (FIB) circulates in the blood and provides the material from which the insoluble fibrin clot is formed during blood coagulation. Fibrinogen is an acute phase reactant that may be a useful marker for infection and inflammation. This ELISA kit can be used to measure Fibrinogen in biological samples.

350

P12799

50,244 Da

Common Pathway 362592!!Complement And Coagulation Cascades Pathway 84312!!Complement And Coagulation Cascades Pathway 484!!Formation Of Fibrin Clot (Clotting Cascade) Pathway 362589!!Formation Of Platelet Plug Pathway 362562!!GRB2:SOS Provides Linkage To MAPK Signaling For Intergrins Pathway 362149!!Hemostasis Pathway 362561!!Integrin AlphaIIb Beta3 Signaling Pathway 362148!!Integrin Cell Surface Interactions Pathway 362147!!Platelet Activation Pathway 362567

Function: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Subunit structure: Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain. Subcellular location: Secreted. Domain: A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. Post-translational modification: Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Sequence similarities: Contains 1 fibrinogen C-terminal domain.

1 x96 Wells

415 USD