MyBioSource

ELISA/assay

Rat CRP ELISA Kit

MBS564107

1 x96 Wells

470 USD

MBS564107

ELISA Kit

Rat CRP ELISA Kit

CRP

Rat CRP

CRP; C-reactive protein; C-reactive protein; pentraxin 1; OTTHUMP00000033307; C-reactive protein, pentraxin-related; N/A

CRP; CRP; PTX1; MGC88244; MGC149895; PTX1

CRP_HUMAN

Rat

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of CRP in Rat Samples. Intended Uses: The CRP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in biological samples of rats.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in humans by Tillet and Frances in 19301. This C-reactive protein (CRP) is so named because it is able to effect precipitation of somatic C-polysaccharide of Streptococcus pneumonia. CRP is an alpha globulin with a mass of 110,000 to 140,000 daltons, and composed of five identical subunits, which are non-covalently assembled as a cyclic pentamer. It is synthesized in the liver and, in humans, is normally present as a trace constituent of serum at levels less that 0.3 mG/dL. The levels in serum rise quickly following acute tissue damage and can reach levels 1000-fold within 24 to 48 hours and also falls very rapidly once the stimulus is removed. It has been proposed that the function of CRP is to aid in complement activation, influence phagocytic cell function, and augment cell mediated cytotoxicity. Investigations over the past few years have shown that quantification of these in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well2.

17975775

P02741

25,039 Da

Classical Antibody-mediated Complement Activation Pathway 106409!!Complement Cascade Pathway 106405!!Creation Of C4 And C2 Activators Pathway 106407!!IL6-mediated Signaling Events Pathway 137932!!Immune System Pathway 106386!!Initial Triggering Of Complement Pathway 106406!!Innate Immunity Signaling Pathway 106387!!Selenium Pathway 198825

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq]

Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Cofactor: Binds 2 calcium ions per subunit. Subunit structure: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits. Subcellular location: Secreted. Tissue specificity: Found in plasma. Induction: The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6. Miscellaneous: This protein owes its name to its ability precipitate pneumococcal C-polysaccharide in the presence of calcium. Sequence similarities: Belongs to the pentaxin family.Contains 1 pentaxin domain. Mass spectrometry: Molecular mass is 23028 Da from positions 19 - 224. Determined by MALDI. Ref.15Molecular mass is 22930 Da from positions 19 - 223. Determined by MALDI. Ref.15

1 x96 Wells

470 USD