ELISA/assay

Rat Clusterin ELISA Kit

MBS564106

1 x96 Wells

490 USD

ELISA Kit

Rat Clusterin ELISA Kit

Clusterin (Apolipoprotein J)

Rat Clusterin (Apolipoprotein J)

clusterin; Clusterin; clusterin; apo-J; NA1/NA2; apolipoprotein J; OTTHUMP00000128311; OTTHUMP00000225247; OTTHUMP00000225248; OTTHUMP00000225249; ku70-binding protein 1; sulfated glycoprotein 2; aging-associated protein 4; complement lysis inhibitor; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2; clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; Testosterone-repressed prostate message 2; TRPM-2Cleaved into the following 2 chains:Clusterin beta chain; Alternative name(s):; ApoJalpha; Complement cytolysis inhibitor a chainClusterin alpha chain; Alternative name(s):; ApoJbeta; Complement cytolysis inhibitor b chain

CLU; CLU; CLI; AAG4; APOJ; KUB1; SGP2; SGP-2; SP-40; TRPM2; TRPM-2; MGC24903; APOJ; CLI; KUB1

CLUS_HUMAN

Rat

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of CLUSTERIN in Rat Samples. Assay Type: Sandwich

Intended Uses: The CLUSTERIN test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CLUSTERIN in biological samples of Rat.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CLUSTERIN present in samples reacts with the anti-CLUSTERIN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CLUSTERIN antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CLUSTERIN. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CLUSTERIN in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CLUSTERIN in the test sample. The quantity of CLUSTERIN in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

42740907

NP_976084.1

NM_203339.2

P10909

52,495 Da

Formation Of Platelet Plug Pathway (106029); Hemostasis Pathway (106028); Platelet Activation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Validated Targets Of C-MYC Transcriptional Repression Pathway (169353)

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.

CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Mitochondrial; Apoptosis. Chromosomal Location of Human Ortholog: 8p21-p12. Cellular Component: Golgi apparatus; extracellular matrix; extracellular space; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; mitochondrial membrane; extracellular region; cytosol; nucleus. Molecular Function: protein binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; misfolded protein binding. Biological Process: platelet activation; response to misfolded protein; release of cytochrome c from mitochondria; positive regulation of nitric oxide biosynthetic process; protein stabilization; positive regulation of apoptosis; response to virus; cell morphogenesis; microglial cell activation; positive regulation of tumor necrosis factor production; negative regulation of protein homooligomerization; activation of NF-kappaB transcription factor; complement activation; reverse cholesterol transport; platelet degranulation; myelin maintenance in the central nervous system; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein import; innate immune response; lipid metabolic process; chaperone-mediated protein complex assembly; blood coagulation; complement activation, classical pathway

1 x96 Wells

490 USD