ELISA/assay

Rat Albumin ELISA Kit

MBS564099

1 x96 Wells

330 USD

ELISA Kit

Rat Albumin ELISA Kit

Albumin

Rat Albumin

albumin; Serum albumin; serum albumin; OTTHUMP00000160370; OTTHUMP00000196832; OTTHUMP00000220435; OTTHUMP00000220436; OTTHUMP00000220438; OTTHUMP00000220439; growth-inhibiting protein 20; cell growth inhibiting protein 42; albumin

ALB; ALB; PRO0883; PRO0903; PRO1341; DKFZp779N1935

ALBU_HUMAN

Rat

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Albumin in Rat Samples. Assay Type: Sandwich

Intended Uses: The Albumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological samples of rats.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Albumin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Albumin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Albumin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Albumin in the test sample. The quantity of Albumin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Albumin (Alb) is an amazing polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.

178344

P02768

69,367 Da

Bile Acid And Bile Salt Metabolism Pathway (106144); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Formation Of Platelet Plug Pathway (106029); HDL-mediated Lipid Transport Pathway (106158); Hemostasis Pathway (106028); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Of Lipids And Lipoproteins Pathway (160976); Platelet Activation Pathway (106034); Platelet Degranulation Pathway (106050)

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq]

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Carrier; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus. Molecular Function: antioxidant activity; toxin binding; protein binding; copper ion binding; enzyme binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding. Biological Process: platelet activation; receptor-mediated endocytosis; sodium-independent organic anion transport; bile acid metabolic process; maintenance of mitochondrion localization; lipoprotein metabolic process; hemolysis by symbiont of host red blood cells; cellular response to starvation; response to mercury ion; response to organic substance; bile acid and bile salt transport; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; transmembrane transport; positive regulation of circadian sleep/wake cycle, non-REM sleep; response to nutrient; negative regulation of apoptosis. Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic

1 x96 Wells

330 USD