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ELISA/assay

Mouse Transferrin ELISA Kit

MBS564087

1 x96 Wells

330 USD

MBS564087

ELISA Kit

Mouse Transferrin ELISA Kit

Transferrin

Mouse Transferrin

transferrin; Serotransferrin; serotransferrin; siderophilin; OTTHUMP00000197155; beta-1 metal-binding globulin; transferrin; Beta-1 metal-binding globulin; Siderophilin

TF; TF; PRO1557; PRO2086; DKFZp781D0156; N/A

TRFE_HUMAN

Mouse

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Transferrin in Mouse Samples. Intended Uses: The Transferrin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Transferrin in biological samples of mice.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Transferrin present in samples reacts with the anti-Transferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Transferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Transferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Transferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Transferrin in the test sample. The quantity of Transferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Transferrin is a metal-combining protein that reversibly bound to acid-soluble iron in plasma. It functions to transport iron to the bone marrow and to tissue storage organs such as the liver. Transferrin also participate in the regulation and control of iron absorption and protects against iron intoxication. Like haptoglobin, the carrier of hemoglobin, transferrin is synthesized in the liver, but unlike haptoglobin transferrin is returned to the circulation after unloading its iron in the reticuloendothelial system. This ELISA kit can be used to measure transferrin in serum, tissue extracts and other biological fluids.

248648

P02787

77,064 Da

EPHB Forward Signaling Pathway 138047!!Formation Of Platelet Plug Pathway 106029!!HIF-1-alpha Transcription Factor Network Pathway 138045!!Hemostasis Pathway 106028!!Iron Uptake And Transport Pathway 187191!!Mineral Absorption Pathway 212237!!Mineral Absorption Pathway 212220!!Platelet Activation Pathway 106034!!Platelet Degranulation Pathway 106050!!Response To Elevated Platelet Cytosolic Ca2+ Pathway 106048

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq]

Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Subunit structure: Monomer. Subcellular location: Secreted. Tissue specificity: Expressed by the liver and secreted in plasma. Polymorphism: Different polymorphic variants of transferrin are known. The sequence shown is the predominant electrophoretic variant (C1 or TF*C1). Involvement in disease: Defects in TF are the cause of atransferrinemia (ATRAF) [. MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Ref.4 Ref.39. Sequence similarities: Belongs to the transferrin family.Contains 2 transferrin-like domains. Sequence caution: The sequence AAF22007.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

1 x96 Wells

330 USD