ELISA/assay

Mouse SAA ELISA Kit

MBS564085

1 x96 Wells

415 USD

ELISA Kit

Mouse SAA ELISA Kit

SAA

Mouse SAA

serum amyloid A protein; Serum amyloid A protein; serum amyloid A protein; SAA; Amyloid fibril protein AA

SAA1; SAA1; SAA

SAA_BOVIN

Mouse

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of SAA in Mouse Samples. Assay Type: Sandwich

Intended Uses: The SAA test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA in biological samples of mice.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

115497340

NP_001068728.1

NM_001075260.1

P35541

14,516 Da

Activated TLR4 Signalling Pathway (362298); Immune System Pathway (362258); Innate Immunity Signaling Pathway (362274); MyD88 Cascade Initiated On Plasma Membrane Pathway (362277); MyD88 Dependent Cascade Initiated On Endosome Pathway (362293); MyD88-independent Cascade Initiated On Plasma Membrane Pathway (362300); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (362299); NFkB And MAP Kinases Activation Mediated By TLR4 Signaling Repertoire Pathway (362302); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (362316); TAK1 Activates NFkB By Phosphorylation And Activation Of IKKs Complex Pathway (362278)

Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Subcellular location: Secreted. Tissue specificity: Expressed by the liver; secreted in plasma. Induction: Upon cytokine stimulation. Post-translational modification: This protein is the precursor of amyloid protein A, which is formed by the removal of residues from the C-terminal end. Involvement in disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Sequence similarities: Belongs to the SAA family.

1 x96 Wells

415 USD