ELISA/assay

Mouse Prealbumin ELISA Kit

MBS564084

1 x96 Wells

520 USD

ELISA Kit

Mouse Prealbumin ELISA Kit

Prealbumin (Transthyretin)

Mouse Prealbumin (Transthyretin)

preproalbumin (serum albumin); Serum albumin; Alpha-livetin

ALB

ALBU_CHICK

Mouse

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Prealbumin in Mouse Samples. Assay Type: Sandwich

Intended Uses: The Prealbumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Prealbumin in biological samples of mice.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Prealbumin present in samples reacts with the anti-Prealbumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-PRE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound PRE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PRE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PRE in the test sample. The quantity of PRE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Prealbumin (PRE) is a tetrameric protein consisting of 4 identical polypeptide chains. It migrates more rapidly than serum albumin on electrophoresis. It can be used as a marker for the nutritional evaluation of patients with a variety of diseases.

63748

P19121

69,918 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Function: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Subcellular location: Secreted. Tissue specificity: Plasma. Allergenic properties: Causes an allergic reaction in human. Binds to IgE. Partially heat-labile allergen that may cause both respiratory and food-allergy symptoms in patients with the bird-egg syndrome. Ref.4. Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.

1 x96 Wells

520 USD