ELISA/assay

Mouse Plasminogen ELISA Kit

MBS564083

1 x96 Wells

470 USD

ELISA Kit

Mouse Plasminogen ELISA Kit

Plasminogen

Mouse Plasminogen

plasminogen; Plasminogen; plasminogen; OTTHUMP00000017544; OTTHUMP00000017545; OTTHUMP00000197002; plasminogen

PLG; PLG; DKFZp779M0222

PLMN_HUMAN

Mouse

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Plasminogen in Mouse Samples. Assay Type: Sandwich

Intended Uses: The Plasminogen test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Plasminogen in biological samples of mice.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Plasminogen present in samples reacts with the anti-Plasminogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-PMG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound PMG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PMG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PMG in the test sample. The quantity of PMG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Plasminogen (PMG) is a glycoprotein produced by the liver. It is the precursor for plasmin, which targets fibrin in the process of dissolution of fibrin blood clots. Plasminogen is present in plasma and most extravascular fluids. The important role of plasminogen in fibrinolytic system makes it an interesting marker for various diseases.

190026

P00747

90,569 Da

Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Diabetes Pathways (105902); Dissolution Of Fibrin Clot Pathway (106061); Formation Of Platelet Plug Pathway (106029); Hemostasis Pathway (106028); Influenza A Pathway (217173)

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.

Plasminogen: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Defects in PLG are the cause of plasminogen deficiency (PLGD). PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. Belongs to the peptidase S1 family. Plasminogen subfamily. Protein type: Secreted, signal peptide; EC 3.4.21.7; Secreted; Motility/polarity/chemotaxis; Protease. Chromosomal Location of Human Ortholog: 6q26. Cellular Component: extracellular space; extrinsic to external side of plasma membrane; cell surface; extracellular region; plasma membrane. Molecular Function: protein domain specific binding; protein binding; serine-type peptidase activity; serine-type endopeptidase activity; apolipoprotein binding; receptor binding. Biological Process: platelet activation; extracellular matrix organization and biogenesis; tissue remodeling; muscle maintenance; myoblast differentiation; negative regulation of cell proliferation; extracellular matrix disassembly; fibrinolysis; platelet degranulation; negative regulation of fibrinolysis; cellular protein metabolic process; proteolysis involved in cellular protein catabolic process; tissue regeneration; positive regulation of fibrinolysis; blood coagulation; transmembrane transport. Disease: Plasminogen Deficiency, Type I

1 x96 Wells

470 USD