ELISA/assay

Human Retinol Binding Protein ELISA Kit

MBS564059

1 x96 Wells

375 USD

ELISA Kit

Human Retinol Binding Protein ELISA Kit

Retinol Binding Protein

retinol-binding protein; Retinol-binding protein 3; retinol-binding protein 3; interstitial retinol-binding protein; retinol-binding protein 3, interstitial; interphotoreceptor retinoid-binding protein; retinol binding protein 3, interstitial; Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein

RBP3; RBP3; IRBP; RBPI; D10S64; D10S65; D10S66; RBP3

RET3_HUMAN

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Retinol Binding Protein in Human Samples. Assay Type: Sandwich

Intended Uses: The Retinol Binding Protein test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Retinol Binding Protein in biological samples of Human.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Retinol Binding Protein (RBP) present in samples reacts with the anti-RBP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-RBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound RBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of RBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of RBP in the test sample. The quantity of RBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

307075

P10745

135,363 Da

The Visual Cycle Pathway (545451); The Visual Cycle Pathway (545294)

Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively. [provided by RefSeq]

RBP3: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. Belongs to the peptidase S41A family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: extracellular space; extracellular region; vesicle. Molecular Function: retinoid binding; serine-type peptidase activity; retinol binding; retinal binding. Biological Process: phototransduction, visible light; visual perception; transport; lipid metabolic process; retinoid metabolic process; proteolysis. Disease: Retinitis Pigmentosa 66; Retinitis Pigmentosa

1 x96 Wells

375 USD