ELISA/assay

Human Myoglobin ELISA Kit

MBS564056

1 x96 Wells

375 USD

ELISA Kit

Human Myoglobin ELISA Kit

Myoglobin

myoglobin; Myoglobin; myoglobin; OTTHUMP00000197919; OTTHUMP00000197921; OTTHUMP00000197922; OTTHUMP00000197935; myoglobin

MB; MB; PVALB; MGC13548

MYG_HUMAN

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Myoglobin in Human Samples. Assay Type: Sandwich

Intended Uses: The Myoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Myoglobin in biological samples of Human.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Myoglobin is a single-chain globular protein with a molecular weight of 16,700 daltons. It contains a heme prosthetic group in the center and is the primary oxygen-carrying pigment of muscle tissues. Myoglobin is released from damaged muscle tissue which is filtered by the kidney. therefore is a sensitive marker for muscle injury.

4885477

NP_005359.1

NM_005368.2

P02144

17,184 Da

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family. Protein type: Carrier. Chromosomal Location of Human Ortholog: 22q13.1. Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity. Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hormone stimulus; brown fat cell differentiation; response to hypoxia; enucleate erythrocyte differentiation

1 x96 Wells

375 USD