ELISA/assay

Human Myeloperoxidase ELISA Kit

MBS564055

1 x96 Wells

390 USD

ELISA Kit

Human Myeloperoxidase ELISA Kit

Myeloperoxidase

Human Myeloperoxidase

myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase

MPO; MPO; MPO

PERM_HUMAN

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Myeloperoxidase in Human Samples. Assay Type: Sandwich

Intended Uses: The Myeloperoxidase test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Myeloperoxidase in serum, plasma of Human.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Myeloperoxidase present in samples reacts with the anti-Myeloperoxidase antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-MPO antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound MPO. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of MPO in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of MPO in the test sample. The quantity of MPO in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Myeloperoxidase (MPO) is synthesized by normal neutrophil and monocyte precursor cells. It is a tetramer consisting of 2 light chains and 2 heavy chains that can mediate microbial killing. There is evidence supporting the fact that Myeloperoxidase may be a very important cardiac and inflammatory biomarker.

4557759

NP_000241.1

NM_000250.1

P05164

83,869 Da

C-MYB Transcription Factor Network Pathway (138073); Folate Metabolism Pathway (198833); IL23-mediated Signaling Events Pathway (138000); Phagosome Pathway (153910); Phagosome Pathway (153859); Selenium Pathway (198825); Amb2 Integrin Signaling Pathway (137945)

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq]

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.11.2.2. Chromosomal Location of Human Ortholog: 17q23.1. Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule. Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding. Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; response to yeast; defense response; response to lipopolysaccharide; response to oxidative stress; defense response to fungus; aging; negative regulation of apoptosis. Disease: Myeloperoxidase Deficiency; Alzheimer Disease

1 x96 Wells

390 USD