Human Lactoferrin ELISA Kit | MyBioSource MBS564053 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Lactoferrin ELISA Kit

catalog :

MBS564053

quantity :

1 x96 Wells

price :

445 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS564053

products type :

ELISA Kit

products full name :

Human Lactoferrin ELISA Kit

products short name :

Lactoferrin

products name syn :

Human Lactoferrin

other names :

lactoferrin; Growth-inhibiting protein 12; lactotransferrin; talalactoferrin; OTTHUMP00000209779; OTTHUMP00000209780; OTTHUMP00000209781; neutrophil lactoferrin; growth-inhibiting protein 12; lactotransferrin

other gene names :

LTF; GIG12; LF; HLF2; GIG12

uniprot entry name :

Q5DSM0_HUMAN

host :

Human

storage stability :

Store at 4 degree C.

other info1 :

Samples: Immunoperoxidase Assay for Determination of Lactoferrin in Human Samples. Assay Type: Sandwich

other info2 :

Intended Uses: The Lactoferrin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Lactoferrin in human biological samples.

products categories :

ELISA Kit

products description :

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Lactoferrin present in samples reacts with the anti-Lactoferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-LF antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound LF. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of LF in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of LF in the test sample. The quantity of LF in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Lactoferrin (LF) is a multifunctional iron-binding protein with antimicrobial activities. It is found in milk and other mucosal secretions. Lactoferrin is known to be part of the innate defense against infection and inflammation and also has function in regulation of iron absorption.

ncbi gi num :

187122

uniprot acc num :

Q5DSM0

ncbi mol weight :

78,384 Da

ncbi pathways :

Amyloids Pathway (366238)

ncbi summary :

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

uniprot summary :

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter. Protein type: EC 3.4.21.-; Protease; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule. Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; ferric iron binding; DNA binding; serine-type endopeptidase activity; iron ion binding. Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; positive regulation of osteoblast differentiation; retinal homeostasis; innate immune response in mucosa; negative regulation of viral genome replication; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of viral reproduction; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of apoptosis

size1 :

1 x96 Wells

price1 :

445 USD

more info or order :

MyBioSource product webpage