ELISA/assay

Human Hemopexin ELISA Kit

MBS564046

1 x96 Wells

375 USD

ELISA Kit

Human Hemopexin ELISA Kit

Hemopexin

hemopexin; Hemopexin; hemopexin; beta-1B-glycoprotein; hemopexin; Beta-1B-glycoprotein

HPX; HPX; HX; FLJ56652; HPX

HEMO_HUMAN

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Hemopexin in Human Samples. Assay Type: Sandwich

Intended Uses: The Hemopexin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin in serum, plasma of Human.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo-genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Hemopexin (HX) is the plasma protein with the highest binding affinity to heme among known proteins. It is mainly expressed in liver and belongs to acute phase reactants, the synthesis of which is induced after inflammation. This ELISA kit can be used to measure Hemopexin in serum, tissue extracts and other biological fluids.

184497

P02790

51,676 Da

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq]

HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.5-p15.4. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; metal ion binding; heme transporter activity. Biological Process: receptor-mediated endocytosis; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of immunoglobulin production; heme transport; positive regulation of tyrosine phosphorylation of Stat1 protein; viral reproduction; hemoglobin metabolic process; cellular iron ion homeostasis; heme metabolic process

1 x96 Wells

375 USD