ELISA/assay

Human Ferritin ELISA Kit

MBS564042

1 x96 Wells

415 USD

ELISA Kit

Human Ferritin ELISA Kit

Ferritin

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Ferritin in Human Samples. Assay Type: Sandwich

Intended Uses: The Ferritin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ferritin in serum or plasma samples of humans.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Ferritin is a water-soluble, iron storage protein. Serum Ferritin level is said to be useful for diagnosis of iron deficiency anemia, metabolism disorder and malignant tumor. Ferritin may also be an acute-phase protein and is often elevated in the course of disease.

1 x96 Wells

415 USD