ELISA/assay

Human beta 2-Microglobulin ELISA Kit

MBS564037

1 x96 Wells

390 USD

ELISA Kit

Human beta 2-Microglobulin ELISA Kit

beta 2-Microglobulin

Human beta 2-Microglobulin

beta-2-microglobulin; Beta-2-microglobulin; beta-2-microglobulin; OTTHUMP00000161912; beta-2-microglobin; beta chain of MHC class I molecules; beta-2-microglobulin

B2M; B2M; B2M

B2MG_HUMAN

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Beta 2-Microglobulin in Human Samples. Assay Type: Sandwich

Intended Uses: The Beta 2-Microglobulin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Beta 2-Microglobulin in biological fluids of Human.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Beta 2-Microglobulin present in samples reacts with the anti-Beta 2-Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-B2M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound B2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of B2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of B2M in the test sample. The quantity of B2M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Beta 2-Microglobulin (B2M) is an 11 kDA protein. It forms the subunit of the MHC class I molecule and associates with the outer membrane of many cells including lymphocytes. It is present in low levels in serum and urine of normal people, but at a higher concentration in patients with renal diseases, kidney transplants and various other inflammatory and infectious conditions.

179318

P61769

13,715 Da

Adaptive Immunity Signaling Pathway (366160); Amyloids Pathway (366238); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); HIV Infection Pathway (105983); Host Interactions Of HIV Factors Pathway (106010)

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; endoplasmic reticulum lumen; early endosome membrane; cytoplasm; extracellular region; plasma membrane; MHC class I protein complex; external side of plasma membrane. Molecular Function: identical protein binding; protein binding. Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent. Disease: Hypoproteinemia, Hypercatabolic

1 x96 Wells

390 USD