ELISA/assay

Human alpha 1-Microglobulin ELISA Kit

MBS564034

1 x96 Wells

390 USD

ELISA Kit

Human alpha 1-Microglobulin ELISA Kit

alpha 1-Microglobulin

Human alpha 1-Microglobulin

alpha-1-microglobulin; Protein AMBP; protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge; alpha-1-microglobulin/bikunin precursor; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in chargeInter-alpha-trypsin inhibitor light chain; ITI-LC; Alternative name(s):; Bikunin; EDC1; HI-30; Uronic-acid-rich protein

AMBP; AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC; AMBP; HCP; ITIL

AMBP_HUMAN

Human

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of Alpha 1 Microglobulin in Human Samples. Assay Type: Sandwich

Intended Uses: The Alpha 1 Microglobulin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 1 Microglobulin in biological fluid of Humans.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 1 Microglobulin present in samples reacts with the anti-Alpha 1 Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1M in the test sample. The quantity of A1M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Alpha 1 Microglobulin (A1M) is a small molecular weight glycoprotein. It is heterogeneous in charge and can occur both as a monomer or a polymer. Alpha 1 Microglobulin is produced in the liver and can be used as indicator for some renal diseases.

579676

P02760

38,999 Da

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq]

AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q32-q33. Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; extracellular region; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; IgA binding; calcium oxalate binding; heme binding; calcium channel inhibitor activity. Biological Process: receptor-mediated endocytosis; negative regulation of JNK cascade; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage

1 x96 Wells

390 USD