Dog CRP ELISA Kit | MyBioSource MBS564014 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Dog CRP ELISA Kit

catalog :

MBS564014

quantity :

1 x96 Wells

price :

480 USD

product information

catalog number :

MBS564014

products type :

ELISA Kit

products full name :

Dog CRP ELISA Kit

products short name :

[CRP]

products name syn :

[Dog CRP]

other names :

[CRP; C-reactive protein; C-reactive protein; pentraxin 1; OTTHUMP00000033307; C-reactive protein, pentraxin-related]

other gene names :

[CRP; CRP; PTX1; MGC88244; MGC149895; PTX1]

uniprot entry name :

CRP_HUMAN

reactivity :

Dog

storage stability :

Store at 4 degree C.

other info1 :

Samples: Immunoperoxidase Assay for Determination of C-Reactive Protein in Dog Samples. Assay Type: Sandwich

other info2 :

Intended Uses: The CRP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in Dog biological samples.

products categories :

ELISA Kit

products description :

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in Human by Tillet and Frances in 19301. This C-reactive protein (CRP) is so named because it is able to effect precipitation of somatic C-polysaccharide of Streptococcus pneumonia. CRP is an alpha globulin with a mass of 110,000 to 140,000 daltons, and composed of five identical subunits, which are non-covalently assembled as a cyclic pentamer. It is synthesized in the liver and, in Human, is normally present as a trace constituent of serum at levels less that 0.3 mG/dL. The levels in serum rise quickly following acute tissue damage and also falls very rapidly once the stimulus is removed. It has been proposed that the function of CRP is to aid in complement activation, influence phagocytic cell function, and augment cell mediated cytotoxicity. Investigations over the past few years have shown that quantification of these in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in Human, but in companion animals and farm herds as well2.

ncbi gi num :

17975775

uniprot acc num :

P02741

ncbi mol weight :

25,039 Da

ncbi pathways :

Classical Antibody-mediated Complement Activation Pathway (106409); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immunity Signaling Pathway (106387); Selenium Pathway (198825)

ncbi summary :

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq]

uniprot summary :

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q23.2. Cellular Component: extracellular space; growth cone; extracellular region; filopodium. Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; virion binding; low-density lipoprotein binding; cholesterol binding; calcium ion binding. Biological Process: protein polymerization; wound healing; defense response to Gram-positive bacterium; response to ethanol; negative regulation of vasodilation; positive regulation of superoxide release; response to lead ion; acute-phase response; response to hypoxia; opsonization; inflammatory response; complement activation, classical pathway; aging

size1 :

1 x96 Wells

price1 :

480 USD