ELISA/assay

Dog Alpha 1-Acid Glycoprotein ELISA Kit

MBS564010

1 x96 Wells

520 USD

ELISA Kit

Dog Alpha 1-Acid Glycoprotein ELISA Kit

Alpha 1-Acid Glycoprotein

Dog Alpha 1-Acid Glycoprotein

alpha-1-acid glycoprotein; Alpha-1-acid glycoprotein; alpha-1-acid glycoprotein; orosomucoid 1; Orosomucoid

Orm1; Orm1; AGP; OMD; Orm; Agpa1

A1AG_RAT

Dog

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of AGP in Dog Samples. Assay Type: Sandwich

Intended Uses: The AGP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring AGP in biological fluid of dogs.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the AGP present in samples reacts with the anti-AGP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-AGP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AGP in the test sample. The quantity of AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Background: AGP is a 52 kDA serine protease inhibitor (serpin) in blood, which protects tissue from enzymes from inflammatory cells, especially elastase. In certain acute phase inflammatory reactions, AGP is elevated in order to limit the damage caused by activated neutrophil granulocytes and their enzyme elastase. Disorders of AGP include AGP deficiency, a hereditary disorder that can lead to severe tissue breakdown during inflammation. This may result in pulmonary emphysema and liver cirrhosis, in severe cases genetic variants of AGP do occur.

16757980

NP_445740.1

NM_053288.1

P02764

23,575 Da

an acute phase reactant protein; may have a role in the acute inflammation response [RGD]

ORM1: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space. Molecular Function: drug binding. Biological Process: response to drug; response to vitamin E; organ regeneration; regulation of immune system process; transport; acute-phase response; response to lipopolysaccharide; response to organic cyclic substance; chronic inflammatory response to antigenic stimulus; acute inflammatory response to antigenic stimulus

1 x96 Wells

520 USD