ELISA/assay

Chicken Ovotransferrin (Conalbumin) ELISA Kit

MBS564009

1 x96 Wells

470 USD

ELISA Kit

Chicken Ovotransferrin (Conalbumin) ELISA Kit

Ovotransferrin

Chicken Ovotransferrin

ovotransferrin; Ovotransferrin; ovotransferrin; serum transferrin; allergen Gal d III; ovotransferrin BB type; ovotransferrin BC type; ovotransferrin CC type; Allergen Gal d III; Conalbumin; Serum transferrin

LTF; TF; TFEW; conalbumin

TRFE_CHICK

Chicken

Store at 4 degree C.

Samples: Biological samples of Chickens. Assay Type: Sandwich

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Ovotransferrin present in samples reacts with the anti-Ovotransferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ovotransferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ovotransferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3' ,5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ovotransferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ovotransferrin in the test sample. The quantity of Ovotransferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

45385813

NP_990635.1

NM_205304.1

P02789

77,777 Da

Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.Transferrin has a bacteriostatic function. Its concentration in avian egg is the highest concentration of any transferrin in vivo. Transferrin in liver is regulated by the iron levels but not in the oviduct. These two forms of transferrin (liver and oviduct) differ only by their carbohydrate composition. Subunit structure: Monomer. Subcellular location: Secreted. Allergenic properties: Causes an allergic reaction in human. Sequence similarities: Belongs to the transferrin family.Contains 2 transferrin-like domains.

1 x96 Wells

470 USD