secondary antibody

Cat IgE ELISA Kit

MBS564005

1 x96 Wells

490 USD

ELISA Kit

Cat IgE ELISA Kit

Cat IgE

immunoglobulin epsilon chain

Cat

Store at 4 degree C.

Samples: Immunoperoxidase Assay for Determination of IgE in Cat Samples. Assay Type: Sandwich

Intended Uses: The IgE test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in biological samples of cats.

ELISA Kit

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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1 x96 Wells

490 USD