catalog number :
MBS560494
products full name :
Affinity Purified Rabbit anti-GFP (GFP Antibody)
products short name :
[GFP]
products name syn :
[Affinity Purified Rabbit anti-Green Fluorescent Protein; anti-GFP]
specificity :
anti-GFP recognizes some variants of recombinant Aequorea green-fluorescent protein.
storage stability :
Lyophilized material should be kept at 4°C until rehydrated and will remain stable for a minumum of 2 years. Rehydrate by adding 1.0mL of reagent quality water to the product vial. Store the liquid solution at 4°C for up to a week; thereafter, aliquots should be prepared and stored frozen. Stable for a minimum of 1 year at -20°C
tested application :
ELISA, Western Blot
app notes :
Suggested working dilutions: . Elisa: 1.0 ug/ml - 1.0 ug/30ml. Blotting: 1.0 ug/ml - 1ug/10ml. Working dilutions must be determined by individual investigator but antibodies have been used with success for Elisa, Dot Blot, Western Blot, and Immunoprecipitation.
other info1 :
Buffer: PBS buffer with non-protein stabilizer
other info2 :
Procedure: Rabbits were immunized with highly purified green-fluorescent protein (GFP) isolated directly from Aequorea victoria. Serum was collected. Antibodies were immunoaffinity purified using the native GFP immobilized on a solid phase.
products categories :
Epitope Tag
products description :
Rabbits were immunized with highly purified green-fluorescent protein (GFP). The resulting antiserum was passed over an antigen containing immunoabsorbant from which the anti-GFP was immunoaffinity purified.
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)