antibody

5-HT (Serotonin) receptor, goat

MBS555344

0.1 mL

455 USD

polyclonal

goat

nonconjugated

western blot, immunohistochemistry

Antibody

5-HT (Serotonin) receptor, goat

[5-HT (Serotonin) receptor]

[5HT]

polyclonal

goat

Human, Mouse, Rat

100ul. Lyophilized

Storage : Dilute with phosphate buffer or Tris buffer at dilutions no higher than 1/10, aliquot and freeze at -15° C or lower. Antibody can be stored for up to six months if handled as described above. It is strongly recommended that the customer perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system as used in our protocol may change the configuration.

Immunohistochemistry; Western Blot

Immunohistochemistry :. Recommended Dilution: 1/400 - 1/800 in PBS/0.3% Triton X-100 – Cy3 Technique; 1/5000 - 1/10,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP . Western Blot :. Recommended Dilution: 1/500 or greater Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Immunohistochemistry:. Antigen: serotonin coupled to BSA with paraformaldehyde. Raised in goat. Control Tissue: Rat hypothalamus, raphe nuclei and spinal cord. Perfusion Fixation: Fixative - 4% paraformaldehyde in 0.1 M Phosphate buffer, p 7.4; 500 mL over approximately 20 minutes. Post Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over approximately 20 s. Post Fixation -1.5 hour at 4 degree C. in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde. Sections: 10um cryostat or 50um vibratome. Antibody dilution: 1/400-1/800 in PBS/0.3% Triton X-100 - Cy3 Technique; 1/5,000 - 1/10,000 in PBS/0.3% Triton X-100 -Bn-SA/HRP Technique. Absorption control: 5-HT/BSA 25ug/mL diluted serum. Incubation on Tissue: 16-24 hours at 4 degree C. Detection System: use Cy3 and Bn-SA/HRP at dilutions recommended by the manufacturers.

Testing Data

Immunogen Sequence: Whole molecule 5HT

Antibodies; 5HT-Serotonins; 5HT (Serotonin)

The histochemical antibody for serotonin is generated in a goat against serotonin conjugated to bovine serum albumin with paraformaldehyde. The antibody has a proven 4+ indirect immunofluorescence staining at a 1/400 - 1/800 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/4000 - 1/10,000 dilution in rat hypothalamus and spinal cord.

0.1 mL

455 USD