catalog number :
MBS555213
products full name :
FMRFamide, Antiserum, Rabbit
products short name :
[FMRFamide]
other names :
[FMRFamide; CG2346 gene product from transcript CG2346-RA; FMRFamide]
products gene name :
[FMRFamide]
other gene names :
[FMRFa; CG2346; dFmrf; dFMRF; dFmrfa; dFMRFa; dFMRFa-1; dFMRFa-2; dFMRFa-3; dFMRFa-4; dFMRFa-5; dFMRFa-6; dFMRFa-7; dFMRFa-8; DmelCG2346; DPKQDFMRF-amide; DPKQDFMRFamide; Drm-FMRF-1; Drm-FMRF-2; Drm-FMRF-3; Drm-FMRF-4; Drm-FMRF-5; Drm-FMRFa1; Drm-FMRFa2; Drm-FMRF]
form :
Whole Serum (with 0.05% sodium azide) Sent in liquid form.
storage stability :
Store frozen. Aliquot as undiluted serum and immediately place at -20ºC. Serum may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20ºC. Repeated freeze/thaw cycles compromise the integrity of the antiserum.
tested application :
Immunohistochemistry (IHC)
app notes :
Immunohistochemistry: 1:200-1:1,000. Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Immunohistochemistry:. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehye-picric acid fixative, and 3) 10% sucrose inPBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
image1 heading :
Testing Data
image2 heading :
Testing Data
other info2 :
Immunogen Sequence: FMRFamide
products categories :
FMRFamide
products description :
First purified from clam ganglia, FMRFamide (Phe-Met-Arg-Phe-NH2) is expressed in both invertebrates and vertebrates. its has been shown to serve various functions in the mammalian nervous system including inhibition of morphine-induced feeding, behavioral effects when administered intrathecally, and to have amnesic effects in rodents. More recently, FMRFamide has been shown to potentiate proton currents and a suggested mode of action is through modulation of ASIC1 and ASIC 3.
ncbi acc num :
NP_523669.2
ncbi gb acc num :
NM_078945.4