Rat PDGFR beta Recombinant | MyBioSource MBS553313 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Rat PDGFR beta Recombinant

catalog :

MBS553313

quantity :

0.05 mg

price :

220 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS553313

products type :

Recombinant Protein

products full name :

Rat PDGFR beta Recombinant

products short name :

[PDGFR beta]

products name syn :

[Rat CD140b Recombinant (PDGF Receptor beta) ; cd-140 PDGFRB; CD140B; MGC74795; PDGFR2; Rhe-PDGFRB PDGF Receptor alpha; pdgf-r]

other names :

[platelet-derived growth factor receptor beta; Platelet-derived growth factor receptor beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1]

products gene name :

[CD140b]

products gene name syn :

[PDGF-R]

other gene names :

[Pdgfrb; Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1]

uniprot entry name :

PGFRB_RAT

sequence length :

1097

purity :

>95%, as determined by SDS-PAGE and HPLC

form :

Recombinant PDGFR beta is supplied as a 0.2 um filtered PBS solution, pH7.2.

storage stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree to 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

other info1 :

Domain: Ig-like C2. Source: HEk293 Cells. Source Note: Optimized DNA sequence encoding extracellular domain of rat PDGFR beta ncluding a C-terminal 6His tag was expressed in HEK293 cells.

other info2 :

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Molecular Weight Note: Recombinant rat PDGF-R beta is a monomer protein consisting of 510 amino acid residue subunits, due to glycosylation migrates as an approximately 100 kDa protein on SDS-PAGE.

products categories :

Receptors CD

products description :

The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (PDGFA, PDGFB, PDGFC, and PDGFD. Ligand binding induces receptor dimerization, enabling autophosphorylation of specific tyrosine residues and subsequent recruitment of a variety of signal transduction molecules. PDGFR regulates normal cellular growth and differentiation, and expression of activated PDGFR promotes oncogenic transformation, suggesting that activating mutations or gene rearrangements could play a role in human tumorigenesis. Numerous in vitro and in vivo studies showed that inhibition of PDGFRA signaling disrupts cancer cell survival in the subset of GISTs with activating PDGFRA mutations.

products references :

hydrophobic matching controls the tilt and stability of the dimeric platelet-derived growth factor receptor (pdgfr) transmembrane segment . J. Biol. Chem., Jul 2012; 287: 26178 - 26186. pdgfr expression and function in fibroblasts derived from pluripotent cells is linked to dna demethylation . J. Cell Sci., May 2012; 125: 2276 - 2287. pdgf-induced migration of vascular smooth muscle cells is inhibited by heme oxygenase-1 via vegfr2 upregulation and subsequent assembly of inactive vegfr2/pdgfr heterodimers . Arterioscler Thromb Vasc Biol, May 2012; 32: 1289 - 1298. a viable mouse pdgfr-beta mutant exhibits cns-restricted pericyte deficiency and blood brain barrier defects . Invest. Ophthalmol. Vis. Sci., Mar 2012; 53: 2558. the leukemia-associated fusion protein tel-platelet-derived growth factor receptor (tel-pdgfr ) inhibits transcriptional repression of ptpn13 gene by interferon consensus sequence binding protein (icsbp) . J. Biol. Chem., Mar 2012; 287: 8110 - 8125.

ncbi gi num :

29789275

ncbi acc num :

NP_113713.1

ncbi gb acc num :

NM_031525.1

uniprot acc num :

Q05030

ncbi mol weight :

122,828 Da

ncbi summary :

binds platelet derived growth factor BB homodimer [RGD, Feb 2006]

uniprot summary :

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent. Protein type: Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Kinase, protein; TK group; PDGFR family

size1 :

0.05 mg

price1 :

220 USD

size2 :

0.2 mg

price2 :

595

size3 :

1 mg

price3 :

2220

more info or order :

MyBioSource product webpage