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protein

Mouse CD80 Recombinant (B7-1)

MBS553255

0.02 mg

220 USD

MBS553255

Recombinant Protein

Mouse CD80 Recombinant (B7-1)

CD80 (B7-1)

Mouse CD80 Recombinant (B7-1) ; CD80; B7-1

T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; Activation B7-1 antigen

CD80

Cd80; B7; B7

CD80_MOUSE

HEk293 Cells

306

>90%, as determined by SDS-PAGE and HPLC

Recombinant mouse CD80 was lyophilized from 0.2um filtered 50mM Tris, 100mM NaCl, pH 8.0.

Recombinant mouse CD80, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months. Avoid repeated freeze/thaw cycles.

Domain: Ig-like C2. Host Note: Optimized DNA sequence encoding extracellular domain of mouse CD80 including a C-terminal 6His tag was expressed in HEK293 cells.

Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug). Molecular Weight Note: Recombinant mouse CD80 is a monomer protein consisting of 220 amino acid residue subunits, due to glycosylation migrates as an approximately 45 kDa protein on SDS-PAGE. Biological Activity: The activity was tested y immobilized recombinant CD80 (1ug/ml) ability to bind recombinant mouse CD28 in functional ELISA.

Receptors CD

CD80 is a monomeric surface protein found on activated APCs. The extracellular region of CD80 consists of two highly glycosylated Ig- like extracellular domains, which have defined regions for binding its ligands, CD28 or CTLA-4, on T cells.One ligand of CD80, CD28, provides critical costimulatory signals to activated T cells to increase cytokine production, cell proliferation, and cell viability, while the other ligand, CTLA-4, may mediate a growth-inhibitory signal.The same regions of CD80 are involved in binding to both CD28 and CTLA-4, but CTLA-4 binds to CD80 with about a 20-fold higher avidity than CD28 does. This suggests that low levels of CTLA-4 expressed on the cell surface can compete for CD80 binding even when CD28 is expressed at much higher levels.

tumor cell programmed death ligand 1-mediated t cell suppression is overcome by coexpression of CD80 . J. Immunol., Jun 2011; 186: 6822 - 6829. toll-like receptor 3 ligands induce CD80 expression in human podocytes via an nf-b-dependent pathway . Nephrol. Dial. Transplant., May 2011; 10.1093/ndt/gfr271. trans-endocytosis of CD80 and cd86: a molecular basis for the cell-extrinsic function of ctla-4 . Science, Apr 2011; 332: 600 - 603. critical role of transcription factor pu.1 in the expression of CD80 and cd86 on dendritic cells . Blood, Feb 2011; 117: 2211 - 2222. enhancement of suboptimal cd8 cytotoxic t cell effector function in vivo using antigen-specific CD80 defective t cells . J. Immunol., Jan 2011; 186: 291 - 304.

112983684

NP_033985.3

NM_009855.2

Q00609

34,590 Da

Function: Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Subcellular location: Membrane; Single-pass type I membrane protein. Tissue specificity: Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies. Developmental stage: Expressed between 4 and 12 hours post-activation. Protein was detected at cell surface at 24 hours and it's expression was maximal from 48 to 72 hours post-activation. Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

0.02 mg

220 USD